a A: the concentration of ethanol aqueous (v/v); B: the ratio of solvent to material;
C: extracting time; D: the temperature of water bath.
b Calibrated by a statistical software, where F0.01(2,2) ¼ 99; F0.05(2,2) ¼ 19; F0.
1(2,2) ¼ 9; F0.25(2,2) ¼ 3.
c Level of significance.
d I, the stage for removal of capsaicinoids; II, the stage for extraction of red
pigment.