During semiconservative DNA replication,for example, the progressive unwinding of the DNA template requires a swivel in the DNA duplex to alleviate the overwinding of the stands ahead of the moving replication fork.Of course,the replication apparatus may simple follow the helical path of the DNA
template strands. However, this soon leads to a second problem of how to unlike the interwound DNA
helices following the completion of DNA synthesis.this decatenation of daughter molecules is absolutely required in the case of circular genomes and plasmids,in which the template strands are
physically linked circles. similar considerations apply to the process of transcription, where the movement of a transcription complex along the DNA template may also produce a local unwinding of the DNA behind and overwinding of the DNA ahead. This may be viewed as the formation of local domains of negatively and positively supercoiled DNA, respectively. Indeed,
the translocation of any complex that forms between the two strands of a DNA duplex
During semiconservative DNA replication,for example, the progressive unwinding of the DNA template requires a swivel in the DNA duplex to alleviate the overwinding of the stands ahead of the moving replication fork.Of course,the replication apparatus may simple follow the helical path of the DNA template strands. However, this soon leads to a second problem of how to unlike the interwound DNA helices following the completion of DNA synthesis.this decatenation of daughter molecules is absolutely required in the case of circular genomes and plasmids,in which the template strands are physically linked circles. similar considerations apply to the process of transcription, where the movement of a transcription complex along the DNA template may also produce a local unwinding of the DNA behind and overwinding of the DNA ahead. This may be viewed as the formation of local domains of negatively and positively supercoiled DNA, respectively. Indeed, the translocation of any complex that forms between the two strands of a DNA duplex
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