Nine tuna dumpling products were purchased at nine retail markets in southern Taiwan. Occurrence of
biogenic amines, histamine-forming bacteria, and adulteration of pork and poultry were determined. This
study showed the high contents of aerobic plate count, total coliform and Escherichia coli in tested tuna
dumpling products. Average content of various biogenic amines in all tested samples was less than
2.0 mg/100 g. Fifteen histamine-producing bacterial strains isolated from tested samples produced 8.7–
466 ppm of histamine in trypticase soy broth supplemented with 1.0% L-histidine (TSBH). Assay of multiplex
polymerase chain reaction (PCR) revealed adulteration rates were 88.9% (8/9) and 33.3% (3/9) for
pork and poultry, respectively, in tuna dumpling. In addition, six samples of tuna dumpling meat were
identified as Thunnus obesus, while other three samples were identified as Thunnus thynnus.