The fatty acid composition of lipids, produced by the yeast strains after 4 days, was examined (Table 2) as it is known to influence the quality of biodiesel produced from biolipids. No huge differences in fatty acid composition were observed between the 12 strains and control strains. Although palmitoleic acid was high in L. starkeyi NBRC 10381, at around about 4.7%, this did not influence the properties of the oil, such as oxidative stability ( Hoekman et al., 2012). In most strains, the major fatty acids were the C18 species; for example, oleic acid (18:1) ranged from 55.43% to 72.95%; stearic acid (18:0) from 6.11% to 25.50% and linoleic acid (18:2) from 3.43% to 27.44%. The percentages of fatty acids remained at a constant level during cultivation. A suitable fatty acid composition for biodiesel production is palmitic (16:0), stearic (18:0), oleic (18:1), linoleic (18:2) and linolenic (18:3) acids ( Knothe, 2009). The 12 selected strains contained these fatty acids at a high ratio, ranging from 92.77% to 97.17%. It can be considered that the fatty acids produced by the selected strains were suitable for biodiesel production.