Procedure
Operating the LI-6400
Put in batteries and attach sensor head to console (take care uncoiling the cable to prevent cable wind).
Unwind lid tension screw to tighten cuvette lid on sensor head.
Attach CO2cartridge: check O-ring is present and in good condition. Place cartridge where it lines up with O-ring, then screw on gently until it feels like it meets, then one last hard and fast screw to break the seal on the cartridge, make sure it is all the way on.
Turn on (using on/off switch).
In main menu, go to configuration menu, go to light source control, go to pick source and select the appropriate setting, press enter and escape to main menu.
Go to new measurements, line 2 and select temperature control. Set block temperature to control at your standard temperature.
In main menu, go to calibration menu, go to zero flow meter, you will have to wait about 10 seconds and then after verifying that the voltage is close to zero (otherwise manually adjust) you can hit OK (F5).
A. Humid conditions (where obtaining a valid H2O zero may be difficult without dry N2gas) In the calibration menu, go to IRGA zeroing, make sure the CO2scrub is all the way on and that the desiccant is all the way off (bypass), then you will hit M to turn Match (Mch) on. Stay in this mode for 20 min to allow IRGAs to warm up. Do not hit auto CO2, auto H2O or auto all. The LI-6400 should only be zeroed when running pure N2gas through the system. Verify good zero values and record.
B. Dry conditions (replace used drierite in scrub column with fresh drierite). In the calibration menu, go to IRGA zeroing, turn the H2O scrub on fully, then you will hit M to turn Match (Mch) on. Stay in this mode for 20 min to allow IRGAs to warm up. Verify good zero values and record. Hit auto H2O and then turn the CO2scrub all the way on and the desiccant all the way off (bypass). Wait for a good zero to be reached (2-5 min) then verify good zero values and record. Hit auto CO2, then exit calibration menu.
- See more at: http://prometheuswiki.publish.csiro.au/tiki-print.php?page=GAS%20EXCHANGE%20PROTOCOL%20FOR%20LI-COR%206400#sthash.pcjwX8fE.dpuf
ProcedureOperating the LI-6400Put in batteries and attach sensor head to console (take care uncoiling the cable to prevent cable wind).Unwind lid tension screw to tighten cuvette lid on sensor head.Attach CO2cartridge: check O-ring is present and in good condition. Place cartridge where it lines up with O-ring, then screw on gently until it feels like it meets, then one last hard and fast screw to break the seal on the cartridge, make sure it is all the way on.Turn on (using on/off switch).In main menu, go to configuration menu, go to light source control, go to pick source and select the appropriate setting, press enter and escape to main menu.Go to new measurements, line 2 and select temperature control. Set block temperature to control at your standard temperature.In main menu, go to calibration menu, go to zero flow meter, you will have to wait about 10 seconds and then after verifying that the voltage is close to zero (otherwise manually adjust) you can hit OK (F5).A. Humid conditions (where obtaining a valid H2O zero may be difficult without dry N2gas) In the calibration menu, go to IRGA zeroing, make sure the CO2scrub is all the way on and that the desiccant is all the way off (bypass), then you will hit M to turn Match (Mch) on. Stay in this mode for 20 min to allow IRGAs to warm up. Do not hit auto CO2, auto H2O or auto all. The LI-6400 should only be zeroed when running pure N2gas through the system. Verify good zero values and record.B. Dry conditions (replace used drierite in scrub column with fresh drierite). In the calibration menu, go to IRGA zeroing, turn the H2O scrub on fully, then you will hit M to turn Match (Mch) on. Stay in this mode for 20 min to allow IRGAs to warm up. Verify good zero values and record. Hit auto H2O and then turn the CO2scrub all the way on and the desiccant all the way off (bypass). Wait for a good zero to be reached (2-5 min) then verify good zero values and record. Hit auto CO2, then exit calibration menu.
- See more at: http://prometheuswiki.publish.csiro.au/tiki-print.php?page=GAS%20EXCHANGE%20PROTOCOL%20FOR%20LI-COR%206400#sthash.pcjwX8fE.dpuf
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