Generally in in vitro cultivation, use is made of growth regulators to stimulate elongation and rooting of shoots, but other factors can also be used as new sources of N in the basal medium, including the organic source. The culture media usually possess ammonium nitrate and potassium nitrate as a source of inorganic N, but the organic N can alsobe used by the plant. The urea may be used in the medium as of the additional N, and has been tested by some authors (Ramos et al., 2009). The type of nutrition is extremely important for the growth and development phases of plants derived from in vitro to support the process of acclimatization (Ramos et al. 2009; Rodrigues et al., 2012).