Thus, prepared as described by Jiang and Li (2001). 0, 5.0, 10.0 or 20.0 g of chitosan was dispersed in 900 ml of distilled water to which 50 ml of glacial acetic acid was added to dissolve the chitosan, to prepare 1.0 L of 0%, 0.5%, 1% or 2% chitosan solutions.