Several previous studies have confirmed that laccase activity and stability under different temperatures and metal ions are positively correlated to its ability to decolorize dyes (Manel et al., 2009, Zilly et al., 2011 and Grassi et al., 2011). Therefore, the results of dye colorization in this study can somewhat reveal that laccase in T. trogii Berk S0301 under the tested conditions possessed attractive properties. In view of its superior decolorization ability in the presence of different metal ions and its high activity at higher temperatures, laccase from T. trogii Berk S0301 may facilitate novel and more efficient bio-catalytic process applications, and the decolorization of 6 other textile dyes by T. trogii Berk S0301 laccase at elevated temperature (60 °C) ( Fig. 3) further broadens its potential applications for the waste dye decolorization.