First-strand cDNA was generated by reverse transcription using random hexamer primers
(Reverse-iTTM-1st Strand Synthesis Kit, ABgene®, Rochester, NY, USA). Relative quantification of CqVg, trypsin and digestive cysteine proteinase 1 expressions was performed with the following primers: CqVg primers: QPCR-F 5-GCTTCCCGGTGGTTAATCCT- 3 and QPCR-R 5-GGGCGGCATGACACACATCT-3; Trypsin primers: F2 5-TGCAAATHE