The PCR products were analyzed after gel electrophoresis. The photographs were critically
examined on the basis of presence (1) or absence (0), size of bands and overall polymorphism of
bands. These were carried out for further investigation. RAPD analysis was then combined to
create a single data matrix. This was used for estimating linkage distance (D) and constructing a
UPGMA (Unweighted Pair Group Method of Arithmetic Means) dendrogram among the varieties
using computer program “Statistica”. Linkage distances were computed from frequencies of
polymorphic markers to estimate genetic relationship between the studied three tomato varieties
using UPGMA (Sneath and Sokal 1973).