Materials and methods: The CQ hexane extract was fractionated sequentially with benzene,dichlor-
omethane, ethyl acetate,and methanol.The examination of CQ extract and its fractions was guided by
bioassays for alkaline phosphatase(ALP) activity during the differentiation of MC3T3-E1 osteoblastic
cells. The cell sweretreatedwithor without the CQ extract and its fractions for aperiod of time, andthen
the stimulatory effect of the alkaline phosphatase enzyme,a bone differentiation marker,was
investigated.The compounds obtained were structurally elucidated using spectroscopic techniques
and re-evaluated for activity during bone differentiation.