After draining and spinning to remove excess water, three different 50 g samples of leaves were individually placed in 1.5 L Whirl-Pak bags containing 200 mL of Dey-Engley (DE) neutralizing broth (Difco); and 25 mL of the drained wash solution was pipetted into a Whirl Pak bag containing 25 mL of double strength DE broth for microbiological analysis. Each treatment combination was replicated two times.