Four different pH of 5.5, 7.5, 8.5 and 9.5 were varied. The pH was adjusted using hydrochloric acid and sodium bicarbonate. Indoor culture condition was maintained inside the room at 25 C with a light intensity of 1588 lux using cool fluorescent light and 24 h photoperiod. The microalgae culture under natural condition was kept outdoor under shade and relied on natural illumination. The initial cell density was 1 105 cells mL 1 for each treatment. Growth parameters in terms of cell density and optical density weremeasured daily. Cells were harvested at their stationary phase by centrifugation at 8000 rpm for 10 min followed by washing twice with sterilize distilled water. Samples were then freeze-dried and kept at 20 C until further analysis. Batch experiments for each microalgae species at various salinities, pH and culture con-ditions were carried out in three replicates. Filtered aeration was provided to all culture flasks throughout the experimental period to provide agitation and facilitate gas exchange.