The biomass was dissolved in a same volume of acetone at reflux temperature for 2.5 h.
After washing for 20 min, the polymer was air dried for 15 min at
30 ◦C. The PHA rate in the biomass was routinely evaluated after
72 h offermentation as yield per liter offermentation substrate and
percentage to the biomass. The polymer was subsequently precipitated three times, from a chloroform solution, on cold methanol
(1:10 v/v chloroform/methanol), washed with methanol, and dried
in a vacuum oven at 30 ◦C.