Evaluation of the effects of selamectin against
adult and immature stages of fleas
(Ctenocephalides felis felis) on dogs and cats
pathogens of both humans and animals, but their true diversity is largely underestimated, and
their
ecology remains to be investigated. Considering their potential threat on human health, it is impor-
tant
to expand our knowledge on the diversity of Chlamydiae, but also to define the host range colonized
by
these bacteria. Thus, using a new pan-Chlamydiales PCR, we analyzed the prevalence of Chlamydiales
DNA
in ticks and fleas, which are important vectors of several viral and bacterial infectious diseases. To
conduct
this study, 1340 Ixodes ricinus ticks prepared in 192 pools were collected in Switzerland and
55
other ticks belonging to different tick species and 97 fleas belonging to different flea species were
harvested
in Algeria. In Switzerland, the prevalence of Chlamydiales DNA in the 192 pools was equal to
28.1%
(54/192) which represents an estimated prevalence in the 1340 individual ticks of between 4.0%
and
28.4%. The pan-Chlamydiales qPCR was positive for 45% (25/55) of tick samples collected in Algeria.
The
sequencing of the positive qPCR amplicons revealed a high diversity of Chlamydiales species. Most
of
them belonged to the Rhabdochlamydiaceae and Parachlamydiaceae families. Thus, ticks may carry
Chlamydiales
and should thus be considered as possible vectors for Chlamydiales propagation to both
humans
and animals.
Evaluation of the effects of selamectin againstadult and immature stages of fleas(Ctenocephalides felis felis) on dogs and catspathogens of both humans and animals, but their true diversity is largely underestimated, andtheirecology remains to be investigated. Considering their potential threat on human health, it is impor-tantto expand our knowledge on the diversity of Chlamydiae, but also to define the host range colonizedbythese bacteria. Thus, using a new pan-Chlamydiales PCR, we analyzed the prevalence of ChlamydialesDNAin ticks and fleas, which are important vectors of several viral and bacterial infectious diseases. Toconductthis study, 1340 Ixodes ricinus ticks prepared in 192 pools were collected in Switzerland and55other ticks belonging to different tick species and 97 fleas belonging to different flea species wereharvestedin Algeria. In Switzerland, the prevalence of Chlamydiales DNA in the 192 pools was equal to28.1%(54/192) which represents an estimated prevalence in the 1340 individual ticks of between 4.0%and28.4%. The pan-Chlamydiales qPCR was positive for 45% (25/55) of tick samples collected in Algeria.Thesequencing of the positive qPCR amplicons revealed a high diversity of Chlamydiales species. Mostofthem belonged to the Rhabdochlamydiaceae and Parachlamydiaceae families. Thus, ticks may carryChlamydialesand should thus be considered as possible vectors for Chlamydiales propagation to bothhumans
and animals.
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