1 g of samples of breast muscle was homogenized within 9 ml of 1.15% KCl. From the solution,100 μl of homogenate were obtained and incubated at 37°C in 80mM Tris maleate buffer (pH 7.4 ) with 5mM FeSO4 (to catalyze lipid peroxidation) in a total volume of 1 ml