After 4 days of storage, the use of acids in the beverages
caused a reduction on the microbial load of E. coli
of about 3 and 6-log10 in the Villanueva and Rojo Vigor
varieties respectively. This reduction level was maintained
or changed minimally during the following days of storage (P > 005). Combining low pH and preservatives,
reductions of 62-log10 on E. coli were achieved in the
first day of storage for beverages of both varieties, and
the same inhibition level was maintained during the following
days (P > 005). Saccharomyces cerevisiae was
reduced 2-log10 at day 14, with only the action of the
acids incorporated in the beverage prepared with Rojo
Vigor variety (Fig. 1a); however, the beverage formulated
with acids and preservatives achieved the same reduction
in less time (4 days). Previous studies have shown the
effectiveness of decreasing pH and of using preservatives
in the inhibition of E. coli in short time periods, coinciding
with the results of this work (Zhao et al. 1993).
After 4 days of storage, the use of acids in the beveragescaused a reduction on the microbial load of E. coliof about 3 and 6-log10 in the Villanueva and Rojo Vigorvarieties respectively. This reduction level was maintainedor changed minimally during the following days of storage (P > 005). Combining low pH and preservatives,reductions of 62-log10 on E. coli were achieved in thefirst day of storage for beverages of both varieties, andthe same inhibition level was maintained during the followingdays (P > 005). Saccharomyces cerevisiae wasreduced 2-log10 at day 14, with only the action of theacids incorporated in the beverage prepared with RojoVigor variety (Fig. 1a); however, the beverage formulatedwith acids and preservatives achieved the same reductionin less time (4 days). Previous studies have shown theeffectiveness of decreasing pH and of using preservativesin the inhibition of E. coli in short time periods, coincidingwith the results of this work (Zhao et al. 1993).
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