500 μL of 0.1 M solution of glucose and 500 μL of the 0.1 M solution of
aniline were mixed with 500 μL of 0.01 M ammonium molybdate in the
microwave transparent tube and diluted up to 2 mL. The reaction was
carried out in a microwave synthesizer for microwave-assisted synthesis
of glucosyl amine and then it was converted to colored complex with
molybdenum. The microwave synthesizer was operated in the range of
100–160 °C using 100 watt microwave power according to the requirement.
No set value was assigned to the pressure and the system was
allowed to adjust in its permissible default value. This value of pressure
was 150 psi which was never obtained. The maximum pressure value in
all these experiments was found to be 138 psi. The mixture of glucose,
aniline and ammonium molybdate was exposed to the microwave
power as per requirement of the experiment. The resulting product
was diluted after microwave treatment up to 10 mL. The blank
contained the same amount of aniline and ammonium molybdate
except glucose and it was diluted up to 10 mL. The absorbance of the
blank as well as standard solutions were measured in the range of
370–800 nm.