which are fundamental in the expression
of diverse genes, could generate diametrically opposed conclusions
giventheir rapid expression levels,low expression levels,and hightranscript instability (data not shown, manuscript in preparation).
In conclusion, the present study provided evidence that the reference genes β-actin, CD81, and YWHAH could be useful for transcript
quantification under several MDBK culture conditions. Importantly, incorporating multiple reference genes provides the most reliable expression pattern after precise normalization.
which are fundamental in the expressionof diverse genes, could generate diametrically opposed conclusionsgiventheir rapid expression levels,low expression levels,and hightranscript instability (data not shown, manuscript in preparation).In conclusion, the present study provided evidence that the reference genes β-actin, CD81, and YWHAH could be useful for transcriptquantification under several MDBK culture conditions. Importantly, incorporating multiple reference genes provides the most reliable expression pattern after precise normalization.
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