the 183 amino-acid residue BSP5
migrates as a major band of ~30 kDa [21, 28], as also observed in the nanopurified sperm
supernatant fractions in the present study. The prevalent (by WB band density) 30 kDa BSP5
species and minor bands in the 50-75 kDa range were present in both pellet and supernatant
fractions after nanopurification.