2.4. Probiotics-in-chocolate with fresh cream or freeze-dried cream Probiotics-in-chocolate with fresh cream was made as follows. Boiled fresh cream(20 g) was added to chocolate(LOTTE Co. Ltd. Saitama, Japan) (50 g) and melted by heating at 40 oc Then 0.5% (wlw) probiotics powder was added and mixed quickly. This chocolate liquid was poured into a tray and cooled at 4 oc for 0.5 h. Probiotics-in-chocolate with freeze-dried cream was made as follows. A chopped chocolate(50 g) was melted by heating at 40 oc. Then freeze-dried cream(10.4 g) and 0.5% (wlw) probiotics powder was added and mixed quickly. This chocolate liquid was poured into a tray and cooled at 4 oc for 1 h.