Finally, 5 µl of PCR product was electrophoresed on a 1.5 per cent agarose gel containing 10 µl of 0.1 per cent ethidium bromide in 0.5×Tris-borate-EDTA buffer. After electrophoresis, amplicons were made visible by UV light irradiation (BIORAD, USA) (Figs 1 & 2).