The values calculated for the standards were entered in a system of coordinates semilogarithmically and analyzed against the AFM1 concentration using Excel (Microsoft, Inc. USA). The AFM1 concentration in mg/L corresponding to the OD of each sample was read from the calibration curve. Three calibration curves were prepared and coefficients of determination (r2) were calculated for each. One curve was prepared for AFM1 in the range between 0.1 and 2 mg/L (r2 ¼ 0.993). A second curve was obtained for the standard solutions for low concentrations of AFM1 (0.05e0.1 mg/L) with r2 ¼ 0.995. A third curve was prepared for AFB1 in the feed at a range of 0.02e0.4 mg/kg (r2 ¼ 0.989). The validation of the ELISA methodswas carried out with the determination of the recoveries and the coefficient of
variation (%CV) for milk and animal feed spiked with three different
concentrations (Table 1).