The method of quality assessment of the oocytes of breeding animals based on morphological criteria
very often leads to unsuccessful in vitro fertilization and pure breeding result. In this paper we present a
spectrophotometric methodology utilizing lab-on-a-chip techniques for optical and subjective characterization
of porcine oocytes. A set of parameters describing the features of oocytes deduced from collected
absorbance spectra of a single oocyte, has been proposed. Differentiation of the spectra of oocytes coming
from different sizes of porcine follicle has been observed. The obtained results are the first step towards an
objective quality assessment of porcine oocytes, which in the future may be extended to other breeding
animals.