After the roots were removed, a fresh L. aromatica plant (2 kg)
was washed and freeze-dried. The freeze-dried sample was
ground into powder using a kitchen milling machine and
passed through a 60-mesh sieve. The powder (100 g) was then
defatted using n-hexane as the solvent. The solideliquid
mixture was filtered with ADVANTEC qualitative filter paper
number 2, and the residue was dried in an oven at 50C for 6
hours. The dried defatted L. aromatica (DFLA) was stored at 4C
prior to extraction.