For qualitative and quantitative analysis, post-chromatographic
derivatization was performed by dipping the developed plate in
p-anisaldehyde sulphuric acid staining solution (for 2 s). Subsequently
to drying for 10 min at room temperature, the plate was heated at 70 °C
for exactly 5 min until violet bands occurred. Detection was carried out
using a photodocumentation system (TLC visualizer, CAMAG AG,
Muttenz, Switzerland) at ultraviolet (UV 366 nm) and white light.
Saponin bands were quantified densitometrically at 545 nm with a slit
dimension of 5.00 × 0.45 mm using a TLC scanner 3 (CAMAG AG,
Muttenz, Switzerland). Evaluation of the densitograms was performed
with the software winCats (CAMAG AG, Muttenz, Switzerland).
For qualitative and quantitative analysis, post-chromatographicderivatization was performed by dipping the developed plate inp-anisaldehyde sulphuric acid staining solution (for 2 s). Subsequentlyto drying for 10 min at room temperature, the plate was heated at 70 °Cfor exactly 5 min until violet bands occurred. Detection was carried outusing a photodocumentation system (TLC visualizer, CAMAG AG,Muttenz, Switzerland) at ultraviolet (UV 366 nm) and white light.Saponin bands were quantified densitometrically at 545 nm with a slitdimension of 5.00 × 0.45 mm using a TLC scanner 3 (CAMAG AG,Muttenz, Switzerland). Evaluation of the densitograms was performedwith the software winCats (CAMAG AG, Muttenz, Switzerland).
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