In vivo evaluation
Powdered roots of S. Collinsiae(200g) were weighed and exhaustively extracted with 70% ethanol by sonication at ambient temperature. The extract was then filtered and adjusted to the concentration equivalent to 250mg plant sample/ml. Flumethrin standard was prepared by diluting Bayticol(conc. 6% w/v of flumethrin) with 70% ethanol to a final concentration of 30ng/ml.