Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were
cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L +NAA at 0.1mg/L) supplemented with different
concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl2), and silver thiosulphate (STS). The addition of AVG,
CoCl2, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The
highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration
frequency with highest number of shoots. This treatment (STS at 5mg/L) produced 40% more shoots per explant compared to
control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl2 the
highest shoot number per explant was found at 1mg/L. Treated with AVG and CoCl2 at 1mg/L increased shoot number by 16 and
12%, respectively, compared to control. Ethylene inhibitors could be used as a possiblemicropropagation and plant transformation
protocol in S. speciosa for plant regenerations.
Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants werecultured on initial shoot regeneration media (MS media with BAP at 2 mg/L +NAA at 0.1mg/L) supplemented with differentconcentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl2), and silver thiosulphate (STS). The addition of AVG,CoCl2, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. Thehighest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regenerationfrequency with highest number of shoots. This treatment (STS at 5mg/L) produced 40% more shoots per explant compared tocontrol followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl2 thehighest shoot number per explant was found at 1mg/L. Treated with AVG and CoCl2 at 1mg/L increased shoot number by 16 and12%, respectively, compared to control. Ethylene inhibitors could be used as a possiblemicropropagation and plant transformationprotocol in S. speciosa for plant regenerations.
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