In previous in vitro and greenhouse tests, we tested eight
Saudi isolates of Trichoderma against M. javanica. Based on
the results of these tests (un-published data), two promising isolates
namely: T. harzianum (isolate-27) and T. viride (isolate-08)
were selected for the present study. The Trichoderma isolates
were first cultured on Potato Dextrose Agar (PDA) on petri
plates. The plates were incubated at 24˚C for 14 days. The
produced conidia were collected from the culture surfaces by
flooding with sterile distilled water and gently scraping the
colony surface with a sterile scrapper