The limit of detection (LOD) was experimentally determined using spiked blank honey extracts with all pesticides. The LOD was defined as the lowest concentration of analyte that could be differentiated of the matrix signal with a signal-to-noise ratio (S/N) higher than 6. The LOQ was based on the trueness and precision data, obtained by recovery determination and was defined as the lowest validated spiked level meeting the requirements of a recovery within the range 70–120% and an RSD 6 20%. Measurement uncertainty (MU) was established according to ISO (International Organization for Standardization)/TS 21748:2004 (International Organization for Standardization, 2004) and EURACHEM guide (Eurachem, 2000).