The cereal suspensions were inoculated with 1% (v/v) pure or
mixed culture (ratio 1:1). All fermentations were carried out at
30 C in a shaker incubator (New Brunswick Scientific CO. INC, NJ,
USA) set at 150 rpm till 28 h under no pH control. Sampling was
done every 2 h at the beginning of fermentation, which was carried
out for 28 h. The samples collected were centrifuged at 4000 rpm
for 7 min and the supernatant was stored at 30 C for chemical
analysis.