Dulbecco’s
modified
Eagle’s medium and Ham’s
nutrient
mixture
containing
fatty acid free
Sigma–Aldrich
penicillin–streptomycin
amphotericin
count
cell viability
testing
performed with a Bürker Counting Chamber (W. Schreck,
Hofheim, Germany) and trypan blue. Bovine oviductal
epithelial cells were diluted to a density of 1 106 cells/mL
and seeded in three different culture systems as described
in the following. The culture mediumwas renewed after 24
hours and subsequently every 48 hours.