Essential oil was obtained by hydro distillation method
(clavenger), and analyzed by gas chromatographymass
spectrometry (GC/MS). Antioxidant activity of
essential oil was conducted using DPPH (2,2-diphenyl-
1-picrylhydrazyl) free-radical scavenging assay (5).
Different concentrations of essential oil were added, at
an equal volume, to methanolic solution of DPPH (100
M). After 15 min at room temperature, the absorbance
was recorded at 517 nm. The experiment was repeated
for three times. BHT and quercetin were used as standard
controls. IC50 values denote the concentration of sample,
which is required to scavenge 50% of DPPH free radicals.