two milliliters overnight inoculum of the above constructed recombinant E.coli strain [E.coli(Calb), E.coli(Tll), E. coli(Calb/Tll), or E.coli(calb-Tll) was added to 100 mL LB medium supplemented with 50 mg/L of kanamycin in a 500 mL Erlenmeyer flask