Analytical methods
Ethanol concentration in the culture
filtrates were determined by gas
chromatography (Shimadzu Co. Ltd., Kyoto,
Japan) using capillary column diameter of
0.32 mm and column length of 30metres withFlame ionization as a detector. Column oven
temperature was 150 °C, detector
temperature was 250°C and Injector port
temperature was 200 °C. Helium was used as
mobile phase with a flux of 1.5 ml/min and
ethanol used an internal standard. The
theoretical yield of ethanol was determined to
be 0.51 g of ethanol per gram of glucose (2
mol of ethanol per 1 mol of glucose). Sugar
concentration was determined by
dinitrosalicylic acid (DNS) method.
19of cellulose/iodine complex and halo zones
were observed in the colored background
after washing. The clear zone around the
mold presented the cellulase producing
capacity of the fungi.20 In this study, 67 pure
fungal isolated from different sources were
tested for cellulase producing strains and 19
isolated were found from 67 that can grow in
CBM agar and efficiency of cellulase
producing strains was measured as a ratio of
the clear zone and the colony width as shown
in Table 1.