In conclusion, a simple 3D tumor cell culture scaffold based on
mulberry silk fibroin of B. mori was fabricated by a simple freeze-
drying method. The results showed the porous microstructure of
our SF scaffold, which made it possible for the cell lines to grow
inside. And then we confirmed that the MKN45 gastric cancer cell
lines were successfully seeded on the SF scaffold and we observed
the cell morphology and distribution after a long time culture. The
findings of our investigate demonstrate that scaffolds based on the
mulberry silk fibroin derived from B. mori have the potential to be
a stable 3D tumor cell culture model, for tumor cytology research
and chemotherapeutic drug sensitivity assay.