In addition, 5AdC (a DNMTs activity inhibitor) (Christman, 2002) significantly decreased the levels of the global DNA methylation by 8.0% compared to control (P < 0.05), and when 0.10 and 1.0 mg/L 2,4-DCP were combined with 0.02 mg/L 5AdC, a significant reduction in the global DNA methylation was also observed (P < 0.05), compared with 2,4-DCP exposure alone (Fig. 6).