The groups were further divided into subgroups of three flasks, andpilocarpine was added immediately to each flask at final concen-trations of 0.05, 0.1, 0.5 and 1 mM. The flasks were incubated in arotatory shaker (110 rpm) at 25◦C, in the dark. The alkaloid uptakewas measured 2 h after its addition. A similar assay was performedwith Na3VO4using final concentrations of 0, 0.5 and 1 mM. Theseexperiments were repeated three times with three replicates each.