การขยายพันธุ์ขมิ้นในหลอดทดลอง
In vitro culture of Curcuma mangga from rhizome bud
Curcuma mangga is one of the important and valuable medicinal crops of the family Zingiberaceae.
This species also has a high potential for essential oil. An efficient protocol for the micropropagation of
C. mangga followed by successful acclimatization to soil was developed. The aseptic rhizome buds
were cultured onto Murashige and Skoog (MS) medium supplemented with different concentrations of
BAP, IAA and NAA for 10 weeks. MS medium supplemented with 9 mg/L BAP was found to be the
optimum for shoot multiplication while MS medium supplemented with 1 mg/L NAA gave the highest
root number. The explants were then subcultured on to MS medium supplemented with different
concentrations of BAP and NAA for 4 weeks. The MS medium supplemented with a combination of 3
mg/L BAP and 1 mg/L NAA gave the highest number of shoot multiplication. The plantlets were
successfully acclimatized with 75% survival rate.