(a) water containing 0É1% (v/v) triÑuoroacetic acid (TFA); (b) water containing 60% (v/v) acetonitrile and 0É07% (v/v) TFA.
For gliadins, the gradient was: 0È1 min 41É7% (b); 1È8 min 41É7È46É2% (b); 8È65 min 46É2È83É0% (b); 65È 69 min 83É0È100% (b); 69È79 min 100% (b); 79È89 min 100È41É7% (b); 89È100 min 41É7% (b). The Ñow rate was 1 ml min~1 and spectrophotometric detection was carried out at 220 nm. Injection volume was 160 ll for each sample. Gliadins were quantiÐed by integration of areas corresponding to solution compositions between 29% and 51% of acetonitril