producers with a bipolar mating system [1]. These species
primarily grow on the stumps of coniferous trees such as pine
(Pinus densiflora), larch (Larix kaempferi), and Korean pine
(Pinus koraiensis). The edible mushroom, Sparassis spp.,
plays significant industrial and economic role as sources of
pharmaceutics, therapeutics, health supplements and
biotechnological products. Phylogenetic analyses of
mushrooms using molecular-based methods have increased
dramatically in the last decade. The phylogenetic relationships
among Sparassis spp. have been studied using nucleotide
sequence data from ribosomal DNAs (rDNA), mitochondrial
rDNAs, and partial RNA polymerase subunit II gene (RPB2) [2–6].
These nucleotide markers as the primary fungal barcode genes
have been used to correctly identify mushroom.