whole flower, each flower was placed in a glass
vessel (148 ml). For the measurement of ethylene
production from various organs, combined organs
from three flowers were placed in a test-tube (14.8
ml), except for petals which were placed in a
50-ml Erlenmeyer flask (66 ml). All vessels were
sealed and kept at 23°C. Two hours later, a 1 ml
gas sample was withdrawn into a syringe and
ethylene concentration determined using