promotes
the aggregation and precipitation of the nanoparticles. Accordingly,
trizma buffer with pH value 7.5 was selected for all
experiments.
Another variable that revealed to influence the fluorescence
intensity was the concentration of trizma buffer solution. Concentrations
between 0.02 and 0.75 mol L1 were evaluated and the
results showed that the maximum value of DF was observed for
0.02 mol L1 which was then chosen for the posterior experiments