Subsequent reactions to generate aroma compounds were carried
out with selected slurries of the fish powder hydrolysates
(FPHs) based on the degree of hydrolysis and free amino acid
content. Aliquots of FPHs (0.2 mL) were mixed, homogenised with a
glucose solution (0.05 mL, 80 mmol/mL) in glass reaction vials and
freeze-dried. Glycerol (500 mL) was added to each freeze-dried
sample to facilitate homogenisation while fish oil (1.5 g/100 g)
was added to some of the samples according to the experimental
design (Table 2). All samples in closed vials were then homogenised at 60 C for 10 min and subsequently heated at 110 C for 30 min to
promote flavour formation. Fish powder hydrolysates without
addition of fish oil and before heating were used as control. All
samples were prepared and analysed in triplicate.