The maximal test was carried out on a treadmill (TRE) (model 10200 ATL, INBRAMED) in order to evaluate the maximal and submaximal VO2 (VO2max; VO2ST) and HR (HRmax; HRST). The protocol began with a 3-minute warm up at a velocity of 6 km·h-1 with 1% inclination. After this, the velocity was increased each 2 minutes by increments of 1 km·h-1, while the inclination remained unchanged.