A SIINT instrument (X- DSC 7000 model, Japan) was used in
order to study the thermal transitions of dough samples during
cooking process.For comparison, samples containing only gluten,
starch, water and salt were run.Samples were weighed onto
aluminum DSC pans and then were hermetically sealed.Pans were
kept 1 h at 25 C and then were heated from 30 C to 90 C at 10 C/
min (together with an empty reference pan). From thermograms
gelatinization enthalpy (DH) and on set (To), peak (Tp) and conclusion
temperatures (Tc) were obtained. After the DSC run, gelatinized
dough samples (in the original sealed pan) were stored at 4 C
for 1, 3, 5, and 7 days for retrogradation studies. After prescribed
storage, samples were allowed to equilibrate at room temperature
for 1 h before being rescanned using DSC with the same heating
program. Each sample was run in triplicate to determine the mean
value.