Juvenile phase tissues derived from in vitro germinated
seeds are the ordinarily explants used for in vitro propaga-
tion of citrus. The plantlets derived from these explants ex-
hibit juvenile traits and require several years of maturation
before they can be evaluated for horticultural and commer-
cial traits (Curtis & Mirkov 2012). On the other hand, be-
cause of the difficulty in obtaining seeds from seedless citrus cultivars e.g. navel sweet oranges, availability of a protocol
for plantlet regeneration from mature explants would be of
great value. The plants without juvenile characteristics
would be ready for filed screening in a shorter period of
time, thus allowing for a more rapid selection process
(Cervera et al. 1998, Kobayashi et al. 2003).