Plant material and adventitious root culture
Adventitious roots were generated from leaves collected from
4-week-old H. perforatum L. plantlets. The leave explants were
cut into small sections (0.5 cm 0.5 cm), transferred to regularstrength
MS (Murashige and Skoog 1962) medium, pH 5.7, supplemented
with B5 vitamins (Gamborg et al., 1968), 3% (w/v) sucrose,
0.5 mg/l indole-3-acetic acid (IAA) and 2.3 mg/l gelrite, and cultured
for 3 weeks at 25 ± 1 C in the dark.