Where, Ai is the initial activity of the free enzyme introduced into
the mixture of Ca-alginate solution assayed using ABTS [2,20-azinobis-(
3-ethylbenzothiazoline-6-sulfonic acid)] as a substrate and
Awash is the laccase activity detected in the curing solution and the
two washing solutions assayed using the same substrate.
All the immobilized laccase beads were kept in distilled water at
4 C until further use.