Preparation of Inoculum: For the preparation of inoculum the culture was plated on PD agar plates. The plates
were incubated at 300
C for 72 hrs until the mycelium sporulates black conidia. Inoculum was produced in 250
ml Erlenmeyer flasks containing 100 ml potato dextrose broth by transferring 2 discs from the PDA plates. The
flasks were incubated for another 72 hrs at 300
C till the mycelial mat develops. This mycelial mat was used as
inoculum in further saccharification experiments.